Journal: Cell

Article Title: SARS-CoV-2 infection triggers profibrotic macrophage responses and lung fibrosis

doi: 10.1016/j.cell.2021.11.033

Figure Lengend Snippet: CD163 + macrophages accumulate in the lung in severe COVID-19 (A) Overview of study design and analyses. CT, computed tomography; BAL, bronchoalveolar lavage; scRNA-seq, single-cell RNA sequencing; snRNA-seq, single-nucleus RNA sequencing; IHC, immunohistochemistry; IF, immunofluorescence microscopy; MELC, multi-epitope ligand cartography; EM, electron microscopy; VCin, inspiratory vital capacity; PBMC, peripheral blood mononuclear cells; IAV, Influenza A virus. (B) Postmortem analysis of consecutive histological sections of non-COVID-19 (left) and COVID-19 autopsy lung samples (right) by hematoxylin and eosin (H&E; top) and CD68 IHC (bottom). Scale bar, 100 μm. (C) IF of CD68 (green) and CD163 (red) in lung tissue autopsy samples of COVID-19 patients and non-COVID-19 controls. Arrows indicate CD68 + CD163 – macrophages, and arrowheads indicate CD68 + CD163 + macrophages. Scale bar, 20 μm. (D) Quantification of CD68 + macrophage density (left) and the proportion of CD163 + macrophages (right) in lung autopsy samples from fifteen donors (as in C). Mann-Whitney test; ∗ p < 0.05. (E) Representative images of consecutive histological sections of lung autopsy samples. H&E (left), CD68 IHC (middle), and SARS-CoV-2 RNA-FISH (right). Arrowheads indicate SARS-CoV-2 RNA-positive macrophages. Scale bars, 50 μm, 25 μm. RNA-FISH, RNA-fluorescence in situ hybridization. (F) Lung autopsy samples of 9 COVID-19 patients were analyzed by MELC with a panel of 22 markers on 19 fields of view (FOVs). Two-dimensional embedding computed by UMAP on 9,684 computationally identified CD45 positive cells (T cells, CD3 + ; B cells, CD20 + ; NK cells, CD56 + ; neutrophils, MRP14 + /CD66b + ; monocytes, MRP14 + /CCR2 + ; macrophages, MRP14 + /HLA-DR + ). (G) Relative proportion (of total CD45 + cells) of cell types in all 19 FOVs (left), and average cell numbers (summary, right).

Article Snippet: CD66b-PE , Miltenyi Biotec , Cat# 130-122-922, N/A.

Techniques: Computed Tomography, RNA Sequencing Assay, Immunohistochemistry, Immunofluorescence, Microscopy, Electron Microscopy, MANN-WHITNEY, Fluorescence, In Situ Hybridization

Journal: Cell

Article Title: SARS-CoV-2 infection triggers profibrotic macrophage responses and lung fibrosis

doi: 10.1016/j.cell.2021.11.033

Figure Lengend Snippet:

Article Snippet: CD66b-PE , Miltenyi Biotec , Cat# 130-122-922, N/A.

Techniques: Immunohistochemistry, Plasmid Preparation, Recombinant, Staining, Lysis, Protease Inhibitor, Mass Spectrometry, Sequencing, Modification, Bicinchoninic Acid Protein Assay, Enzyme-linked Immunosorbent Assay, Software

Journal: Inflammation

Article Title: Alpha-1 Antitrypsin Mediates Spontaneous Resolution of Acute Gouty Inflammation Via Inhibiting Caspase 3/GSDME-dependent Macrophage Pyroptosis

doi: 10.1007/s10753-025-02424-z

Figure Lengend Snippet: AAT is elevated in acute gout, especially in synovial macrophages. A , Serum AAT levels in healthy controls (HC, n = 47), gout patients in intermittent phase (Intermittent, n = 37), and patients with acute gout (Acute, n = 41). B , Relative mRNA expression of SERPINA1 in PBMCs from healthy controls (HC, n = 42), gout patients in intermittent phase (Intermittent, n = 40), and patients with acute gout (Acute, n = 41) was detected by RT-qPCR. C , AAT levels in synovial fluids from patients with acute gout ( n = 4) and osteoarthritis ( n = 4) detected by ELISA. D , Immunofluorescence microscopy revealing the expression of AAT in CD68 + synovial macrophages, CD66b + synovial neutrophils, CD117 + synovial mast cells, and Vimentin + synovial fibroblasts, in synovial fluids from patients with acute gout and osteoarthritis. Scale bar, 150 μm. E , Relative mRNA expression of SRPINA1 detected by RT-qPCR in THP-1-derived macrophages treated with or without MSU crystals ( n = 3). F , Western blotting analysis and quantitative comparison of AAT in THP-1-derived macrophages after treatment with or without MSU crystals ( n = 3). β-actin, loading control. G , Concentration of AAT in supernatants from THP-1-derived macrophages after treatment with or without MSU crystals ( n = 3–4). Data are presented as median (range; A - C ) and mean ± SEM ( E - G ). Statistical significance was determined by Kruskal-Wallis test ( A and B ) and unpaired t test ( C and E - G ). * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. PBMCs, peripheral blood monocytes. ACTB , Actin Beta . OA, osteoarthritis

Article Snippet: Antibody against CD66b (55428) was from Signalway antibody.

Techniques: Expressing, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Microscopy, Derivative Assay, Western Blot, Comparison, Control, Concentration Assay